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EFFECT OF DIFFERENT CELL DISRUPTION METHODS IN EXTRACTION OF SOLUBLE PROTEINS FROM MICROALGAE CHLOROCOCCUM SP.
TALITA ABRANTE LEITE, WALLADY DA SILVA BARROSO, ANDRé LUIS COELHO DA SILVA, JOSé OSVALDO BESERRA CARIOCA, GERMANA NOGUEIRA BEZERRA, JéSSICA FERNANDES DE SOUZA, BARTOLOMEU WARLENE SILVA DE SOUZA
3 BIOTECNOLOGIA E INOVAçõES
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Microalgae; Chlorococcum sp.; microalgae cell disruption
Last years, many of the studies were concentrated on lipid extraction for fuel purposes, neglecting the potential of microalgae to produce proteins and other high-value components. However, new research have been doing with the purpose to add value to others microalgae's components such as proteins. The present study focuses on evaluating the effect of different cell disruption techniques on protein extractability of microalgae Chlorococcum sp. First, the microalgae was cultivated in 50 % sea water-based NPK culture medium, and were grown in batch mode in a 4 L indoor tubular air-lift photo-bioreactor at 25 °C. The culture was incubated phototrophically with 140.5 μmol photons m−2s−1 illumination and a light/dark cycle of 13/11 h for 15 days. Microalgae were harvested during the exponential growth phase and concentrated by centrifugation, and then submitted to different cell disruption techniques: manual grinding, ultrasonication, alkaline treatment, and thermolysis. The biomass concentration of the paste was 20–24% dry weight. After conducting cell disruption, the profile and concentration of soluble proteins extracted in were determined (by SDS-PAGE and Bradford method, respectively) for all conditions. As result, it was found that the aqueous solutions containing most protein concentration followed the order: ultrasonication> chemical treatment > manual grinding >thermolysis.